The illuminated plant cell.
نویسنده
چکیده
The past decade has provided biologists with a palette of genetically encoded, multicolored fluorescent proteins. The living plant cell turned into a 'coloring book' and today, nearly every text-book organelle has been highlighted in scintillating fluorescent colors. This review provides a concise listing of the earliest representative fluorescent-protein probes used to highlight various targets within the plant cell, and introduces the idea of using the numerous multicolor, subcellular probes for the development of an early intracellular response profile of plants.
منابع مشابه
Cellulose accumulation and a cellulose synthase gene are responsible for cell aggregation in the cyanobacterium Thermosynechococcus vulcanus RKN.
A thermophilic cyanobacterium, Thermosynechococcus vulcanus RKN, exhibits cell aggregation under low temperature illuminated conditions as a means of physiological acclimation to avoid excess light stress. The cell aggregation was dispersed with cellulase treatment. We developed a method to quantify small amounts of cellulose by partial cellulose purification followed by quantitation of liberat...
متن کاملEffects of phosphorylation on phosphoenolpyruvate carboxykinase from the C4 plant Guinea grass.
In the C4 plant Guinea grass (Panicum maximum), phosphoenolpyruvate carboxykinase (PEPCK) is phosphorylated in darkened leaves and dephosphorylated in illuminated leaves. To determine whether the properties of phosphorylated and non-phosphorylated PEPCK were different, PEPCK was purified to homogeneity from both illuminated and darkened leaves. The final step of the purification procedure, gel ...
متن کاملTwo NADPH:Protochlorophyllide Oxidoreductases in Barley: Evidence for the Selective Disappearance of PORA during the Light-Induced Greening of Etiolated Seedlings.
Chlorophyll synthesis in barley is controlled by two different light-dependent NADPH:protochlorophyllide oxidoreductases, termed PORA and PORB. PORA is present abundantly in etioplasts but selectively disappears soon after the beginning of illumination. This negative light effect is mediated simultaneously at three different levels. First, the concentration of porA mRNA declines drastically dur...
متن کاملRelationship between Photosynthesis and Respiration: The Effect of Carbohydrate Status on the Rate of CO(2) Production by Respiration in Darkened and Illuminated Wheat Leaves.
The rate of dark CO(2) efflux from mature wheat (Triticum aestivum cv Gabo) leaves at the end of the night is less than that found after a period of photosynthesis. After photosynthesis, the dark CO(2) efflux shows complex dependence on time and temperature. For about 30 minutes after darkening, CO(2) efflux includes a large component which can be abolished by transferring illuminated leaves to...
متن کاملPlastid transcription activity and DNA copy number increase early in barley chloroplast development.
Plastid transcription activity and DNA copy number were quantified during chloroplast development in the first foliage leaf in dark-grown and illuminated barley (Hordeum vulgare L.) seedlings. Primary foliage leaves of seedlings given continuous illumination from 2 days post-imbibition reached a final mean length of 15 centimeters at 6.5 days, whereas primary leaves of dark-grown seedlings requ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Trends in plant science
دوره 12 11 شماره
صفحات -
تاریخ انتشار 2007